Confirmation of soybean plastid rRNAs by formaldehyde denaturing agarose gel electrophoresis
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چکیده
منابع مشابه
Agarose gel electrophoresis.
MATERIALS 6x Gel-loading buffer Agarose solutions (please see Step 3) DNA samples DNA size standards Samples of DNAs of known size are typically generated by restriction enzyme digestion of a plasmid or bacteriophage DNA of known sequence. Alternatively, they are produced by ligating a monomer DNA fragment of known size into a ladder of polymeric forms. DNA staining solution For a discussion of...
متن کاملAgarose gel electrophoresis.
Agarose gel electrophoresis is used to separate DNA or RNA molecules based upon their size.
متن کاملAlkaline agarose gel electrophoresis.
INTRODUCTION Alkaline agarose gels are run at a pH that is sufficiently high to denature double-stranded DNA. The denatured DNA is maintained in a single-stranded state and migrates through the alkaline gel as a function of its size. Alkaline agarose gels are used chiefly to measure the size of first and second strands of cDNA (Construction of cDNA Libraries Stage 1: Synthesis of First-strand c...
متن کاملScreening for Amyloid Aggregation by Semi-Denaturing Detergent-Agarose Gel Electrophoresis
Amyloid aggregation is associated with numerous protein misfolding pathologies and underlies the infectious properties of prions, which are conformationally self-templating proteins that are thought to have beneficial roles in lower organisms. Amyloids have been notoriously difficult to study due to their insolubility and structural heterogeneity. However, resolution of amyloid polymers based o...
متن کاملPrinciples of Nucleic Acid Separation by Agarose Gel Electrophoresis
Agarose gel electrophoresis is a routinely used method for separating proteins, DNA or RNA. (Kryndushkin et al., 2003). Nucleic acid molecules are size separated by the aid of an electric field where negatively charged molecules migrate toward anode (positive) pole. The migration flow is determined solely by the molecular weight where small weight molecules migrate faster than larger ones (Samb...
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ژورنال
عنوان ژورنال: Genetics and Molecular Research
سال: 2014
ISSN: 1676-5680
DOI: 10.4238/2014.october.27.3